Amantadine resistance among porcine H1N1, H1N2, and H3N2 influenza A viruses isolated in Germany between 1981 and 2001.
Identifieur interne : 000502 ( Main/Exploration ); précédent : 000501; suivant : 000503Amantadine resistance among porcine H1N1, H1N2, and H3N2 influenza A viruses isolated in Germany between 1981 and 2001.
Auteurs : Michaela Schmidtke [Allemagne] ; Roland Zell ; Katja Bauer ; Andi Krumbholz ; Christina Schrader ; Jochen Suess ; Peter WutzlerSource :
- Intervirology [ 0300-5526 ] ; 2006.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Allemagne (MeSH), Amantadine (pharmacologie), Animaux (MeSH), Antiviraux (pharmacologie), Données de séquences moléculaires (MeSH), Embryon de poulet (MeSH), Gènes viraux (MeSH), Infections à Orthomyxoviridae (médecine vétérinaire), Infections à Orthomyxoviridae (virologie), Influenzavirus A (effets des médicaments et des substances chimiques), Influenzavirus A (génétique), Influenzavirus A (isolement et purification), Lignée cellulaire (MeSH), Maladies des porcs (virologie), Méthode des plages virales (MeSH), Phylogenèse (MeSH), Protéines de la matrice virale (génétique), Résistance virale aux médicaments (génétique), Sous-type H1N1 du virus de la grippe A (effets des médicaments et des substances chimiques), Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H3N2 du virus de la grippe A (effets des médicaments et des substances chimiques), Substitution d'acide aminé (MeSH), Suidae (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Influenzavirus A, Sous-type H1N1 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A.
- génétique : Influenzavirus A, Protéines de la matrice virale, Résistance virale aux médicaments, Sous-type H1N1 du virus de la grippe A.
- isolement et purification : Influenzavirus A.
- médecine vétérinaire : Infections à Orthomyxoviridae.
- pharmacologie : Amantadine, Antiviraux.
- virologie : Infections à Orthomyxoviridae, Maladies des porcs.
- Alignement de séquences, Allemagne, Animaux, Données de séquences moléculaires, Embryon de poulet, Gènes viraux, Lignée cellulaire, Méthode des plages virales, Phylogenèse, Substitution d'acide aminé, Suidae, Séquence d'acides aminés.
- Wicri :
- geographic : Allemagne.
English descriptors
- KwdEn :
- Amantadine (pharmacology), Amino Acid Sequence (MeSH), Amino Acid Substitution (MeSH), Animals (MeSH), Antiviral Agents (pharmacology), Cell Line (MeSH), Chick Embryo (MeSH), Drug Resistance, Viral (genetics), Genes, Viral (MeSH), Germany (MeSH), Influenza A Virus, H1N1 Subtype (drug effects), Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H3N2 Subtype (drug effects), Influenzavirus A (drug effects), Influenzavirus A (genetics), Influenzavirus A (isolation & purification), Molecular Sequence Data (MeSH), Orthomyxoviridae Infections (veterinary), Orthomyxoviridae Infections (virology), Phylogeny (MeSH), Sequence Alignment (MeSH), Swine (MeSH), Swine Diseases (virology), Viral Matrix Proteins (genetics), Viral Plaque Assay (MeSH).
- MESH :
- chemical , genetics : Viral Matrix Proteins.
- chemical , pharmacology : Amantadine, Antiviral Agents.
- geographic : Germany.
- drug effects : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Influenzavirus A.
- genetics : Drug Resistance, Viral, Influenza A Virus, H1N1 Subtype, Influenzavirus A.
- isolation & purification : Influenzavirus A.
- veterinary : Orthomyxoviridae Infections.
- virology : Orthomyxoviridae Infections, Swine Diseases.
- Amino Acid Sequence, Amino Acid Substitution, Animals, Cell Line, Chick Embryo, Genes, Viral, Molecular Sequence Data, Phylogeny, Sequence Alignment, Swine, Viral Plaque Assay.
Abstract
This study was designed to gain insight into amantadine susceptibility of porcine influenza A viruses isolated in Germany between 1981 and 2001. The 12 studied H1N1, H1N2, and H3N2 porcine influenza virus strains were isolated in chicken eggs and passaged once in MDCK cells. Plaque reduction assays were applied to examine virus susceptibility to amantadine. Genotyping was used to confirm drug resistance. In the results of these antiviral studies, only 3 of the 12 isolates were shown to be amantadine-susceptible. All resistant strains contained the AA substitutions G16E, S31N, and R77Q in the membrane protein 2 (M2). Additionally, L27A was detected in two H1N1 strains. S31N and/or L27A are well-known amino acid substitutions in M2 that confer amantadine resistance. The role of the pig as an intermediate host of avian and human influenza A viruses, the possible involvement of genetic reassortment, and the high incidence of naturally amantadine-resistant porcine influenza A viruses suggest a real risk of emergence of amantadine resistant human viruses. Therefore, drug susceptibility monitoring appears to be warranted for effective application of those drugs.
DOI: 10.1159/000094244
PubMed: 16809934
Affiliations:
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(MeSH)</term><term>Orthomyxoviridae Infections (veterinary)</term><term>Orthomyxoviridae Infections (virology)</term><term>Phylogeny (MeSH)</term><term>Sequence Alignment (MeSH)</term><term>Swine (MeSH)</term><term>Swine Diseases (virology)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Plaque Assay (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Allemagne (MeSH)</term><term>Amantadine (pharmacologie)</term><term>Animaux (MeSH)</term><term>Antiviraux (pharmacologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Embryon de poulet (MeSH)</term><term>Gènes viraux (MeSH)</term><term>Infections à Orthomyxoviridae (médecine vétérinaire)</term><term>Infections à Orthomyxoviridae (virologie)</term><term>Influenzavirus A (effets des médicaments et des substances chimiques)</term><term>Influenzavirus A (génétique)</term><term>Influenzavirus A (isolement et purification)</term><term>Lignée cellulaire (MeSH)</term><term>Maladies des porcs (virologie)</term><term>Méthode des plages virales (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Protéines de la matrice virale (génétique)</term><term>Résistance virale aux médicaments (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (effets des médicaments et des substances chimiques)</term><term>Sous-type H1N1 du virus de la grippe A (génétique)</term><term>Sous-type H3N2 du virus de la grippe A (effets des médicaments et des substances chimiques)</term><term>Substitution d'acide aminé (MeSH)</term><term>Suidae (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Amantadine</term><term>Antiviral Agents</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Germany</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenzavirus A</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Influenzavirus A</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drug Resistance, Viral</term><term>Influenza A Virus, H1N1 Subtype</term><term>Influenzavirus A</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Influenzavirus A</term><term>Protéines de la matrice virale</term><term>Résistance virale aux médicaments</term><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenzavirus A</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Influenzavirus A</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Amantadine</term><term>Antiviraux</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à Orthomyxoviridae</term><term>Maladies des porcs</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Orthomyxoviridae Infections</term><term>Swine Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Amino Acid Substitution</term><term>Animals</term><term>Cell Line</term><term>Chick Embryo</term><term>Genes, Viral</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Alignment</term><term>Swine</term><term>Viral Plaque Assay</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Allemagne</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Embryon de poulet</term><term>Gènes viraux</term><term>Lignée cellulaire</term><term>Méthode des plages virales</term><term>Phylogenèse</term><term>Substitution d'acide aminé</term><term>Suidae</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Allemagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study was designed to gain insight into amantadine susceptibility of porcine influenza A viruses isolated in Germany between 1981 and 2001. The 12 studied H1N1, H1N2, and H3N2 porcine influenza virus strains were isolated in chicken eggs and passaged once in MDCK cells. Plaque reduction assays were applied to examine virus susceptibility to amantadine. Genotyping was used to confirm drug resistance. In the results of these antiviral studies, only 3 of the 12 isolates were shown to be amantadine-susceptible. All resistant strains contained the AA substitutions G16E, S31N, and R77Q in the membrane protein 2 (M2). Additionally, L27A was detected in two H1N1 strains. S31N and/or L27A are well-known amino acid substitutions in M2 that confer amantadine resistance. The role of the pig as an intermediate host of avian and human influenza A viruses, the possible involvement of genetic reassortment, and the high incidence of naturally amantadine-resistant porcine influenza A viruses suggest a real risk of emergence of amantadine resistant human viruses. Therefore, drug susceptibility monitoring appears to be warranted for effective application of those drugs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16809934</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>22</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0300-5526</ISSN><JournalIssue CitedMedium="Print"><Volume>49</Volume><Issue>5</Issue><PubDate><Year>2006</Year></PubDate></JournalIssue><Title>Intervirology</Title><ISOAbbreviation>Intervirology</ISOAbbreviation></Journal><ArticleTitle>Amantadine resistance among porcine H1N1, H1N2, and H3N2 influenza A viruses isolated in Germany between 1981 and 2001.</ArticleTitle><Pagination><MedlinePgn>286-93</MedlinePgn></Pagination><Abstract><AbstractText>This study was designed to gain insight into amantadine susceptibility of porcine influenza A viruses isolated in Germany between 1981 and 2001. The 12 studied H1N1, H1N2, and H3N2 porcine influenza virus strains were isolated in chicken eggs and passaged once in MDCK cells. Plaque reduction assays were applied to examine virus susceptibility to amantadine. Genotyping was used to confirm drug resistance. In the results of these antiviral studies, only 3 of the 12 isolates were shown to be amantadine-susceptible. All resistant strains contained the AA substitutions G16E, S31N, and R77Q in the membrane protein 2 (M2). Additionally, L27A was detected in two H1N1 strains. S31N and/or L27A are well-known amino acid substitutions in M2 that confer amantadine resistance. The role of the pig as an intermediate host of avian and human influenza A viruses, the possible involvement of genetic reassortment, and the high incidence of naturally amantadine-resistant porcine influenza A viruses suggest a real risk of emergence of amantadine resistant human viruses. Therefore, drug susceptibility monitoring appears to be warranted for effective application of those drugs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schmidtke</LastName><ForeName>Michaela</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Virology and Antiviral Therapy, Medical Centre of the Friedrich Schiller University Jena, Jena, Germany. michaela.schmidtke@med.uni-jena.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zell</LastName><ForeName>Roland</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Bauer</LastName><ForeName>Katja</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Krumbholz</LastName><ForeName>Andi</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Schrader</LastName><ForeName>Christina</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Suess</LastName><ForeName>Jochen</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Wutzler</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>06</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Intervirology</MedlineTA><NlmUniqueID>0364265</NlmUniqueID><ISSNLinking>0300-5526</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C488937">M2 protein, Influenza A virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014763">Viral Matrix Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>BF4C9Z1J53</RegistryNumber><NameOfSubstance UI="D000547">Amantadine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000547" MajorTopicYN="N">Amantadine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019943" MajorTopicYN="N">Amino Acid Substitution</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002642" MajorTopicYN="N">Chick Embryo</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D024882" MajorTopicYN="N">Drug Resistance, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005814" MajorTopicYN="N">Genes, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005858" MajorTopicYN="N" Type="Geographic">Germany</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053122" MajorTopicYN="N">Influenza A Virus, H3N2 Subtype</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029521" MajorTopicYN="N">Influenzavirus A</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009976" MajorTopicYN="N">Orthomyxoviridae Infections</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013552" MajorTopicYN="N">Swine</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013553" MajorTopicYN="N">Swine Diseases</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014763" MajorTopicYN="N">Viral Matrix Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010948" MajorTopicYN="N">Viral Plaque Assay</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>06</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>7</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>7</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16809934</ArticleId><ArticleId IdType="pii">94244</ArticleId><ArticleId IdType="doi">10.1159/000094244</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Bauer, Katja" sort="Bauer, Katja" uniqKey="Bauer K" first="Katja" last="Bauer">Katja Bauer</name><name sortKey="Krumbholz, Andi" sort="Krumbholz, Andi" uniqKey="Krumbholz A" first="Andi" last="Krumbholz">Andi Krumbholz</name><name sortKey="Schrader, Christina" sort="Schrader, Christina" uniqKey="Schrader C" first="Christina" last="Schrader">Christina Schrader</name><name sortKey="Suess, Jochen" sort="Suess, Jochen" uniqKey="Suess J" first="Jochen" last="Suess">Jochen Suess</name><name sortKey="Wutzler, Peter" sort="Wutzler, Peter" uniqKey="Wutzler P" first="Peter" last="Wutzler">Peter Wutzler</name><name sortKey="Zell, Roland" sort="Zell, Roland" uniqKey="Zell R" first="Roland" last="Zell">Roland Zell</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Schmidtke, Michaela" sort="Schmidtke, Michaela" uniqKey="Schmidtke M" first="Michaela" last="Schmidtke">Michaela Schmidtke</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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